About US

Next Gen DNA Lab is a high-complexity molecular diagnostics laboratory. We are one of only a few high-complexity molecular DNA laboratories in the entire nation. Our core values are patient focused, integrity, respect and teamwork.

We incorporate the use of innovative technologies and complex techniques, such as Real-Time PCR, which allows our laboratory to achieve an outstanding diagnostic accuracy of over 99% - a percentage significantly more precise than traditional forms of lab testing. With Real-Time PCR you will also receive your results within a 24 hour timespan, and that includes antibiotic resistance results, which can typically take up to 5-7 days with traditional laboratory tests.

We are on the forefront of revolutionizing medicine by helping physicians precisely diagnose patients, early on. This allows doctors to successfully map treatment plans that are as unique as each patients' individual DNA.

Our tests are accurate and results are back in 24-48 hours. We also offer customized panels based upon a physician’s request. This allows us to build tests based on what physicians see during their day-to-day practice of medicine.

Our Team

Our Experts

Next Gen DNA Lab is equipped with the latest technology as well as the experts who effectively use it to deliver rapid, accurate and actionable results to patients.

Collectively, our leadership team has many years of clinical, laboratory and research experience.

NEXT GEN DNA Lab in collaboration with NCF Diagnostics & DNA Technologies, Alachua FL, USA. Our lab utilizes Real Time PCR – Quant Studio 5 for pathogen identification, quantification and detection of antibiotic resistance.

Our team of experts includes:

• Accessions - Professionals who receive patient samples at our facility.

• Molecular Technologists - Specialists who process the actual lab tests.

• Researchers - Experts who are on the forefront of investing/interpreting data and designing new DNA tests.

The Team

• Ramakrishna Pai J, MD (Microbiology),
  Lab Director

• Bharathi. MD.,
  Consultant Pathologist

• Ashwin Kumar MD.,
  Consultant Biochemist

• N.S. Usha, M.Sc., Ph.D (Biochemistry)
  Consultant Biochemist

• Vijayashree N, M.Sc., Ph.D (Microbiology)
  Consultant Microbiologist

• N.S. Venkatasubbaiah, B.Sc, LLB
  Legal Consultant

• N.S.T Rao, B.com, LLB, CA
  Financial Advisor

• Dr. Vikas V, M.S (Surgery)
  Associate Lab Director

• Dr. Vikhyath L, M.D (Pediatrics)
  Associate Lab Director

• Anjana N, M.Sc. (Microbiology)
  Lab Technologist

• Deepa Rajesh, M.Sc. (Biochemistry)
  Lab Technologist

• Rajani K.A, M.Sc (Microbiology)
  Lab Technologist

• Natesh N.V., D.M.E, Dip. Bio Med Engg
  Marketing and Lab Operation

• N.S Ramaswamy. B.com, LLB.,
  Accountant

• Vinay Vasudeva Rao, BE, MS.,
  IT Consultant

• Archana C.R, B.E.,
  IT Technology

• N.S. Susheela, M.A,
  Lab Manager

• N.S. Saroja, B.Sc, M.Ed
  Office Manager

About PCR

What is a PRIMER?

A PRIMER is a small piece of a single-strand of a DNA sequence. NCFDNA specializes in developing primers to be used with PCR Technology.

How is a PRIMER used?

Primers are used to initiate a Polymerise Chain Reaction.

What is a Polymerase Chain Reaction?

A PCR (Polymerise Chain Reaction) is a process used to turn a SINGLE copy of a GENE into more than a BILLION copies. It was developed in 1983 by Kary Mullis, who was later presented with a Nobel Prize.

Why do we need multiple copies of genes?

• To detect the presence of matching genes in pathogens

• To look for variations in genes that may cause disease

• To analyse patient samples for identification in forensics

• To produce many copies of genes for genetic engineering

What is involved in a Polymerase Chain Reaction?

• DNA extracted from a specimen

• Primer (single strand of a manufactured DNA sequence)

• HEAT

• Polymerase Enzyme (naturally occurring)

What are the stages of PCR?

• DENATURING – A sample of DNA from a specimen is heated to cause separation of the double-stranded structure creating two separate single-strand templates.

• ANNEALING – PRIMERS are introduced that attach to each single strand template of DNA making each one double-stranded again. The temperature is lowered to allow for this reaction to happen.

• EXTENDING – The temperature is raised again, but only to 72 degrees this time. The polymerase enzyme is added to the solution, attaches to the new double stranded structure and begins to add nucleotides to lengthen the DNA strands.